Dissecting the cellular architecture of breast cancer brain metastases reveals prognostically distinct immune landscapes
(1) Jassowicz L (2) Feng F (3) Warta R (4) Heinicke F (5) Wllner A (6) Briesch H (7) Sor F (8) Soller J (9) Lotsch C (10) Wong JKL (11) Park CG (12) Aubert N (13) Scheer M (14) Hu Z (15) Knoll M (16) Schlegel J (17) Frank L (18) Dao Trong P (19) Jungwirth G (20) Nohman AI (21) Maa§ K (22) Grabe N (23) Barthel M (24) Butz S (25) Lulay C (26) Zucknick M (27) Bortolomeazzi M (28) Sant P (29) Thewes V (30) Michel L (31) Fremd C (32) Schneeweiss A (33) Sun C (34) Rippe K (35) Abdollahi A (36) von Deimling A (37) Unterberg A (38) Krieg SM (39) Mallm JP (40) Lichter P (41) Zapatka M (42) Seiffert M (43) Herold-Mende C
Jassowicz, Feng, and Warta et al. used tissue cytometry, bulk and single-nucleus RNAseq, flow cytometry, and spatial transcriptomics to profile 156 human breast cancer (BC) brain metastases (BCBM) and map functionally and clinically distinct immune landscapes. Enrichment of intratumoral CD8+ TRM-like cells expressing high activation and effector markers, and the presence of tertiary lymphoid structures (TLSs) in BCBM were identified as two favorable immune landscapes associated with improved prognosis. TRM and TLS gene signatures predicted both overall survival in independent BCBM and primary BC cohorts, and response to ICB across cohorts with various cancer types.
Contributed by Shishir Pant
(1) Jassowicz L (2) Feng F (3) Warta R (4) Heinicke F (5) Wllner A (6) Briesch H (7) Sor F (8) Soller J (9) Lotsch C (10) Wong JKL (11) Park CG (12) Aubert N (13) Scheer M (14) Hu Z (15) Knoll M (16) Schlegel J (17) Frank L (18) Dao Trong P (19) Jungwirth G (20) Nohman AI (21) Maa§ K (22) Grabe N (23) Barthel M (24) Butz S (25) Lulay C (26) Zucknick M (27) Bortolomeazzi M (28) Sant P (29) Thewes V (30) Michel L (31) Fremd C (32) Schneeweiss A (33) Sun C (34) Rippe K (35) Abdollahi A (36) von Deimling A (37) Unterberg A (38) Krieg SM (39) Mallm JP (40) Lichter P (41) Zapatka M (42) Seiffert M (43) Herold-Mende C
Jassowicz, Feng, and Warta et al. used tissue cytometry, bulk and single-nucleus RNAseq, flow cytometry, and spatial transcriptomics to profile 156 human breast cancer (BC) brain metastases (BCBM) and map functionally and clinically distinct immune landscapes. Enrichment of intratumoral CD8+ TRM-like cells expressing high activation and effector markers, and the presence of tertiary lymphoid structures (TLSs) in BCBM were identified as two favorable immune landscapes associated with improved prognosis. TRM and TLS gene signatures predicted both overall survival in independent BCBM and primary BC cohorts, and response to ICB across cohorts with various cancer types.
Contributed by Shishir Pant
ABSTRACT: Breast cancer brain metastases (BCBM) are a severe condition with high demand for improved personalized treatment, but a comprehensive understanding of BCBM immune-microenvironment heterogeneity and susceptibility to immunotherapy is lacking. Here, we multimodally profile the immune niche in a clinically well-annotated cohort of 156 BCBM applying tissue cytometry, bulk and single nuclei RNA-sequencing, flow cytometry, and spatial transcriptomics, complemented by functional studies in patient-derived models. Integrative analyses reveal two immune landscapes predicting prolonged patient survival and that are not deducible from paired primary tumors: 1) BCBM with a high proportion of CD8(+) tissue-resident-like memory T cells as major players of tumor immune control. 2) BCBM containing tertiary lymphoid structures. Surrogate signatures of these landscapes are prognostic in independent BCBM and primary breast cancer cohorts, are associated with fewer metastases, and predict immunotherapy response. Our work provides critical insights into anti-tumor immunity in BCBM and identifies novel biomarkers with translational relevance.
Author Info:
(1) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Divis
ion of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address: lena.jassowicz@med.uni-heidelberg.de. (2) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. (3) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (4) Department of Biostatistics, Oslo Centre for Biostatistics and Epidemiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. (5) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (6) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany; Faculty of Biosciences, Heidelberg University, Heidelberg, Germany. (7) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (8) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (9) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (10) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. (11) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. (12) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. (13) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (14) Division Immune Regulation in Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany. (15) Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, Heidelberg, Germany. (16) Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, Heidelberg, Germany. (17) Division of Chromatin Networks, German Cancer Research Center (DKFZ) and Bioquant, Heidelberg, Germany. (18) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (19) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (20) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (21) Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and Hopp Children's Cancer Center at the NCT Heidelberg (KiTZ), Heidelberg, Germany. (22) Hamamatsu Tissue Imaging and Analysis Center, BioQuant, University of Heidelberg, Heidelberg, Germany. (23) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (24) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (25) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (26) Department of Biostatistics, Oslo Centre for Biostatistics and Epidemiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway; Oslo Centre for Biostatistics and Epidemiology, Research Support Services, Oslo University Hospital, Oslo, Norway. (27) Single Cell Open Lab, German Cancer Research Center (DKFZ), Heidelberg, Germany. (28) Single Cell Open Lab, German Cancer Research Center (DKFZ), Heidelberg, Germany. (29) National Center for Tumor Diseases (NCT), NCT Heidelberg, a Partnership Between the German Cancer Research Center (DKFZ), the University Hospital Heidelberg (UKHD), The Heidelberg Medical Faculty of the Heidelberg University, and the Thorax Clinic Heidelberg, Heidelberg, Germany. (30) Department of Medical Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (31) National Center for Tumor Diseases (NCT), NCT Heidelberg, a Partnership Between the German Cancer Research Center (DKFZ), the University Hospital Heidelberg (UKHD), The Heidelberg Medical Faculty of the Heidelberg University, and the Thorax Clinic Heidelberg, Heidelberg, Germany. (32) National Center for Tumor Diseases (NCT), NCT Heidelberg, a Partnership Between the German Cancer Research Center (DKFZ), the University Hospital Heidelberg (UKHD), The Heidelberg Medical Faculty of the Heidelberg University, and the Thorax Clinic Heidelberg, Heidelberg, Germany; Department of Medical Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany; Department of Gynecology and Obstetrics, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (33) Division Immune Regulation in Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany. (34) Division of Chromatin Networks, German Cancer Research Center (DKFZ) and Bioquant, Heidelberg, Germany. (35) Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, Heidelberg, Germany. (36) Department of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (37) Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (38) Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. (39) Single Cell Open Lab, German Cancer Research Center (DKFZ), Heidelberg, Germany. (40) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany; National Center for Tumor Diseases (NCT), NCT Heidelberg, a Partnership Between the German Cancer Research Center (DKFZ), the University Hospital Heidelberg (UKHD), The Heidelberg Medical Faculty of the Heidelberg University, and the Thorax Clinic Heidelberg, Heidelberg, Germany. (41) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. (42) Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address: m.seiffert@dkfz-heidelberg.de. (43) Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany. Electronic address: h.mende@med.uni-heidelberg.de.
Citation: Cancer Cell 2026 Jun 8 44:1290-1311.e16 Epub04/27/2026
