Connolly et al. investigated the dynamics of antitumor, stem-like CD8+ T cells (TSL) in a mouse model of KP lung adenocarcinoma (LUAD). Although T cells became excluded from tumors over time, TCF1+PD-1+ TSL were consistently detected in tumors and local lymph nodes (LNs) and TCR clonotypes were shared between locations. Relative to intratumoral TSL, which acquired markers of TCR signaling and terminal differentiation, LN TSL remained less differentiated. Blocking lymphocyte migration with FTY720 reduced TSL in tumors, but not LNs. In a LUAD patient dataset, TSL were dominant in LNs, while exhausted T cells predominated in tumors.
Contributed by Alex Najibi
ABSTRACT: “Stem-like” TCF1+ CD8+ T (TSL) cells are necessary for long-term maintenance of T cell responses and the efficacy of immunotherapy, but, as tumors contain signals that should drive T cell terminal differentiation, how these cells are maintained in tumors remains unclear. In this study, we found that a small number of TCF1+ tumor-specific CD8+ T cells were present in lung tumors throughout their development. Yet, most intratumoral T cells differentiated as tumors progressed, corresponding with an immunologic shift in the tumor microenvironment (TME) from “hot” (T cell inflamed) to “cold” (non–T cell inflamed). By contrast, most tumor-specific CD8+ T cells in tumor-draining lymph nodes (dLNs) had functions and gene expression signatures similar to TSL from chronic lymphocytic choriomeningitis virus infection, and this population was stable over time despite the changes in the TME. dLN T cells were the developmental precursors of, and were clonally related to, their more differentiated intratumoral counterparts. Our data support the hypothesis that dLN T cells are the developmental precursors of the TCF1+ T cells in tumors that are maintained by continuous migration. Last, CD8+ T cells similar to TSL were also present in LNs from patients with lung adenocarcinoma, suggesting that a similar model may be relevant in human disease. Thus, we propose that the dLN TSL reservoir has a critical function in sustaining antitumor T cells during tumor development and in protecting them from the terminal differentiation that occurs in the TME
Author Info: (1) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (2) Department of Neuroscience, Yale University School of Medicine, New Haven, CT, US
Author Info: (1) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (2) Department of Neuroscience, Yale University School of Medicine, New Haven, CT, USA. (3) Computational Biology and Bioinformatics Program, Yale University, New Haven, CT, USA. (4) Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA. (5) Computational Biology and Bioinformatics Program, Yale University, New Haven, CT, USA. (6) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (7) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (8) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (9) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (10) Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA. (11) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (12) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (13) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (14) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (15) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (16) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (17) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (18) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA. (19) Department of Biostatistics, Yale School of Public Health, New Haven, CT 06510, USA. (20) Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA. Versiti Blood Research Institute, Milwaukee, WI 53213, USA. (21) Department of Genetics and Computer Science, Yale University School of Medicine, New Haven, CT, USA. (22) Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA.
