(1) Xiao Y (2) Mao L (3) Yang QC (4) Wang S (5) Wu ZZ (6) Wan SC (7) Zhang MJ (8) Sun ZJ

Oral oncology

OBJECTIVES: CD103(+)CD8(+)T cells is a subtype of T cells with excellent tumor killing ability and it could response to immune checkpoint blockade therapy in several types of cancer, but the phenotype, role and molecular mechanism CD103(+)CD8(+)T cells in the OSCC still unclear. MATERIALS AND METHODS: The distribution and phenotype of CD103(+)CD8(+)T cells were investigated by performing multiplexed immunohistochemistry on human OSCC tissue microarray and flow cytometric analysis of fresh OSCC tumor-infiltrating lymphocytes (TILs). By in vivo use of anti-CD103 monoclonal antibody (mAb) in the 4MOSC1 tumor-bearing mouse model, CD103(+)CD8(+)T cell infiltration and cytotoxicity was clarified. RESULTS: The majority of CD8(+)T cells in both human and animal OSCC intra-tumoral region were CD103(+)CD8(+)T cells with high expression levels of cytotoxic molecules, which can be impaired by CD103 blockade. In addition, combined use of anti-CD103 mAb with anti-CTLA-4 mAb displayed impaired immune checkpoint blockade therapy efficiency. CONCLUSION: CD103(+)CD8(+)T cells are the major intra-tumoral subset of CD8(+)T cells in both animal and human OSCC, and that CD103(+)CD8(+)T cells demonstrate remarkable tumor-infiltrating and tumor-killing properties, thereby CD103(+)CD8(+)T cells may critical for anti-CTLA-4 immunotherapy in OSCC.

Author Info: (1) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology

Author Info: (1) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (2) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (3) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (4) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (5) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (6) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (7) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. (8) The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China; Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: sunzj@whu.edu.cn.

Citation: Oral Oncol 2023 Feb 6 138:106331 Epub02/06/2023

Link to PUBMED: http://www.ncbi.nlm.nih.gov/pubmed/36753904

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