CD96 has recently been shown to be a potent immune checkpoint molecule in mice but a similar role in humans is unknown. In this study we provide a detailed map of CD96 expression across human lymphocyte lineages, the kinetics of CD96 regulation upon T cell activation and co-expression with other conventional and emerging immune checkpoint molecules. We show that CD96 is predominantly expressed by T cells and has a unique lymphocyte expression profile. CD96(high) T cells exhibited distinct effector functions upon activation. Of note, CD96 expression highly correlated with T cell markers in primary and metastatic human tumors and was elevated on antigen-experienced T cells and tumor infiltrating lymphocytes. Collectively, these data demonstrate that CD96 may be a promising immune checkpoint to enhance T cell function against human cancer and infectious disease. This article is protected by copyright. All rights reserved.
Author Info: (1) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (2) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (3) QIMR Berghofer Med
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Author Info: (1) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (2) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (3) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (4) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (5) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. Faculty of Medicine, The University of Queensland, Brisbane, QLD, 4029, Australia. Centre for Biodiscovery and Molecular Development of Therapeutics, AITHM, James Cook University, Cairns, QLD, 4870, Australia. (6) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. Faculty of Medicine, The University of Queensland, Brisbane, QLD, 4029, Australia. Centre for Biodiscovery and Molecular Development of Therapeutics, AITHM, James Cook University, Cairns, QLD, 4870, Australia. (7) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (8) Discipline of Pathology, School of Medical Sciences, Charles Perkins Centre, University of Sydney, NSW, 2006, Australia. (9) Department of Microbiology and Immunology, University of Otago, Dunedin, 9010, New Zealand. (10) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (11) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (12) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. Faculty of Medicine, The University of Queensland, Brisbane, QLD, 4029, Australia. (13) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. (14) QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. Faculty of Medicine, The University of Queensland, Brisbane, QLD, 4029, Australia. Centre for Biodiscovery and Molecular Development of Therapeutics, AITHM, James Cook University, Cairns, QLD, 4870, Australia. Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, CF14 4XN, UK. Griffith University, Brisbane, QLD, 4111, Australia.
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