Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing
July 26, 2017
The more data you collect, the more details, and hopefully insights, you’ll uncover; that’s why Zheng et al. performed single-cell RNA sequencing and T cell receptor (TCR) profiling on over 5,000 individual T cells isolated from the tumors, adjacent normal tissue, and peripheral blood of six patients with hepatocellular carcinoma (HCC). From the...
Personalized Neoantigen Vaccines for High-risk Melanoma Patients
July 19, 2017
The ability to induce de novo, potent, and truly tumor-specific T cell responses as shown in two independent studies with different vaccine formats by Ott & Hu et al. (Ott&Hu) and Sahin et al. (Sahin) has the potential to transform cancer vaccination. T cells directed against neoantigens, which arise from non-silent somatic mutations...
Mitochondrial dysregulation and glycolytic insufficiency functionally impair CD8 T cells infiltrating human renal cell carcinoma
July 12, 2017
Despite extensive CD8+ T cell infiltration, clear cell renal cell carcinoma (ccRCC) responds only moderately to checkpoint blockade therapy, suggesting other immunosuppressive mechanisms. One mechanism by which tumors can avoid effector T cell function is by metabolically inhibiting the tumor-infiltrating lymphocytes (TIL). Siska et al. used a combination of mass and flow cytometry...
Depletion of tumor-associated macrophages with a CSF-1R kinase inhibitor enhances antitumor immunity and survival induced by DC immunotherapy
July 5, 2017
Malignant mesothelioma is a cancer characterized by a significant stromal component that is dominated by macrophages. Tumor-associated macrophages (TAMs) are supported by macrophage-colony stimulating factor (M-CSF), which not only enhances TAM survival and proliferation, but also their recruitment towards tumors and the expression of a pro-tumor phenotype. TAMs are linked to immune suppression...